esterase substrate

By Targets:	Biochemical Assay Reagents (3) Endogenous Metabolite (1) Fluorescent Dye (6)
By Research Areas:	Metabolic Disease (1) Neurological Disease (1)

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-131131

5-CFDA-AM	Fluorescent Dye	Others
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell .

HY-135036

4-Methylumbelliferyl butyrate	Others	Others
4-Methylumbelliferyl butyrate (4-MUB) is a coumarin-based fluorogenic substrate used for the identification of M. catarrhalis C4- esterase. 4-Methylumbelliferyl butyrate can converse to the blue-emissive 4-methylumbelliferone (4-MU; HY-N0187) .

HY-52112

BCDA 5-bromo-4-chloroindoxyl acetate	Fluorescent Dye	Others
BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase .

HY-D1678

5(6)-Carboxynaphthofluorescein diacetate	Fluorescent Dye	Others
5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .

HY-W275616

4-Methylumbelliferyl nonanoate	Others	Others
4-Methylumbelliferyl nonanoate is a fluorogenic substrate of esterases. 4-Methylumbelliferyl nonanoate can be hydrolyzed to 4-methylumbelliferone with bright blue fluorescence .

HY-D0719

Fluorescein Diacetate 2 Publications Verification 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein	Biochemical Assay Reagents	Others
Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.

HY-145127

ETZ C3-CA-DTZ	Fluorescent Dye	Neurological Disease
ETZ (C3-CA-DTZ) is a promising luciferase substrate (prosubstrate) activatable in vivo by nonspecific esterase to enhance the brain delivery of the luciferin .

HY-P2831

Esterase, pig liver CESs	Endogenous Metabolite	Metabolic Disease
Esterase, pig liver (CESs), namely carboxylate hydrolases, are widely distributed in nature, commonly found in mammalian liver, and often used in biochemical research. Esterase catalyzes the hydrolysis of a variety of endogenous and exogenous substrates, including esters, thioesters, carbamates, and amides, hydrolyzing carboxylic acid esters to the corresponding alcohols and carboxylic acids .

HY-W099563

4-Nitrophenyl stearate	Biochemical Assay Reagents	Others
4-Nitrophenyl stearate, which is an ester formed by the condensation of stearic acid and 4-nitrophenol, is commonly used as a substrate for enzymatic assays, where the hydrolysis of ester bonds by esterase and lipase can be measured by absorbance or ratio In addition, 4-Nitrophenyl stearate has been used as a model compound to study the enzymatic activity and selectivity of lipases and esterases from various sources. The long hydrophobic tail of the molecule makes it suitable for use in lipophilic Good solubility in the environment makes it a useful probe for studying lipid metabolism.

HY-W320032

5-Bromo-1H-indol-3-yl octanoate	Fluorescent Dye	Others
5-Bromo-1H-indol-3-yl octanoate is a chromogenic substrate for esterase with C8 activity. 5-Bromo-1H-indol-3-yl octanoate yields a blue precipitate when cleaved.

HY-D1739

DDAO phosphate diammonium	Fluorescent Dye	Others
DDAO phosphate diammonium is a fluorescent phosphatase substrate. DDAO phosphate diammonium has tunable excitation wavelength (600-650nm) and long emission wavelength (λem=656nm). DDAO phosphate diammonium can be used to detect the activity of different enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin and esterase.

HY-W014449

4-Nitrophenyl butyrate	Biochemical Assay Reagents	Others
4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, thus giving it a yellow color. This compound is commonly used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Thus, the rate of color change can be used to determine enzyme activity. In addition, 4-Nitrophenyl butyrate can also be used as organic synthesis reagent and dye intermediate.

Cat. No.	Product Name	Type

HY-131131

5-CFDA-AM	Fluorescent Dyes/Probes
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell .

5-CFDA-AM

Fluorescent Dyes/Probes

5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell .

HY-52112

BCDA 5-bromo-4-chloroindoxyl acetate	Chromogenic Substrates
BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase .

HY-D0719

Fluorescein Diacetate 2 Publications Verification 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein	Dyes Enzyme Substrates
Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.

HY-D1678

5(6)-Carboxynaphthofluorescein diacetate	Fluorescent Dyes/Probes
5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .

HY-W320032

5-Bromo-1H-indol-3-yl octanoate	Chromogenic Substrates
5-Bromo-1H-indol-3-yl octanoate is a chromogenic substrate for esterase with C8 activity. 5-Bromo-1H-indol-3-yl octanoate yields a blue precipitate when cleaved.

HY-D1739

DDAO phosphate diammonium	Fluorescent Dyes/Probes
DDAO phosphate diammonium is a fluorescent phosphatase substrate. DDAO phosphate diammonium has tunable excitation wavelength (600-650nm) and long emission wavelength (λem=656nm). DDAO phosphate diammonium can be used to detect the activity of different enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin and esterase.

Cat. No.	Product Name	Type

HY-D0719

Fluorescein Diacetate 2 Publications Verification 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein	Dyes Enzyme Substrates
Fluorescein diacetate is a cell permeable esterase-substrate. Fluorescein diacetate can be used as a fluorogenic substrate for hGSTP1-1.

HY-W099563

4-Nitrophenyl stearate	Biochemical Assay Reagents
4-Nitrophenyl stearate, which is an ester formed by the condensation of stearic acid and 4-nitrophenol, is commonly used as a substrate for enzymatic assays, where the hydrolysis of ester bonds by esterase and lipase can be measured by absorbance or ratio In addition, 4-Nitrophenyl stearate has been used as a model compound to study the enzymatic activity and selectivity of lipases and esterases from various sources. The long hydrophobic tail of the molecule makes it suitable for use in lipophilic Good solubility in the environment makes it a useful probe for studying lipid metabolism.

4-Nitrophenyl stearate

Biochemical Assay Reagents

4-Nitrophenyl stearate, which is an ester formed by the condensation of stearic acid and 4-nitrophenol, is commonly used as a substrate for enzymatic assays, where the hydrolysis of ester bonds by esterase and lipase can be measured by absorbance or ratio In addition, 4-Nitrophenyl stearate has been used as a model compound to study the enzymatic activity and selectivity of lipases and esterases from various sources. The long hydrophobic tail of the molecule makes it suitable for use in lipophilic Good solubility in the environment makes it a useful probe for studying lipid metabolism.

HY-W014449

4-Nitrophenyl butyrate	Biochemical Assay Reagents
4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, thus giving it a yellow color. This compound is commonly used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Thus, the rate of color change can be used to determine enzyme activity. In addition, 4-Nitrophenyl butyrate can also be used as organic synthesis reagent and dye intermediate.

4-Nitrophenyl butyrate

Biochemical Assay Reagents

4-Nitrophenyl butyrate consists of butyric acid chains esterified with 4-nitrophenol groups, thus giving it a yellow color. This compound is commonly used as a substrate in enzyme assays to measure esterase and lipase activity. When these enzymes cleave the ester bond, the nitrophenol group is released and the color changes from yellow to orange. Thus, the rate of color change can be used to determine enzyme activity. In addition, 4-Nitrophenyl butyrate can also be used as organic synthesis reagent and dye intermediate.

Cat. No.	Product Name	Target	Research Area

HY-P4772

Me-CO-Lys(Cbo)-Gly-Arg-pNA

Peptides Others

Me-CO-Lys(Cbo)-Gly-Arg-pNA is a substrate for C1 esterase .

MCE Japan Authorized Agent ナミキ商事株式会社コスモ・バイオ株式会社
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Inhibitors & Agonists

Fluorescent Dye

Biochemical Assay Reagents

Peptides